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Genotype : gk-817d10-025709 gk-817d10-025709 gk-817d10-025722 (AT3G46020 AT3G46020 AT3G46030) A. thaliana

Description/Method  gk-817d10-025709, gk-817d10-025709, gk-817d10-025722: PCR was performed on DNA from Arabidopsis thaliana plants (T1) which were transformed with the T-DNA from vector pAC106. The lines contain one or more T-DNA insertions. The DNA fragment(s) resulting from the PCR were directly sequenced to determine the genomic sequence flanking the insertion. Sequences displaying significant similarity to the A. thaliana nuclear genome sequence were processed for submission. T-DNA derived sequences were removed; PCR was performed on DNA from Arabidopsis thaliana plants (T1) which were transformed with the T-DNA from vector pAC106. The lines contain one or more T-DNA insertions. The DNA fragment(s) resulting from the PCR were directly sequenced to determine the genomic sequence flanking the insertion. Sequences displaying significant similarity to the A. thaliana nuclear genome sequence were processed for submission. T-DNA derived sequences were removed; PCR was performed on DNA from Arabidopsis thaliana plants (T1) which were transformed with the T-DNA from vector pAC106. The lines contain one or more T-DNA insertions. The DNA fragment(s) resulting from the PCR were directly sequenced to determine the genomic sequence flanking the insertion. Sequences displaying significant similarity to the A. thaliana nuclear genome sequence were processed for submission. T-DNA derived sequences were removed Type  polymorphic_genetic_context
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